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Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Fauland, A; Köfeler, H; Trötzmüller, M; Knopf, A; Hartler, J; Eberl, A; Chitraju, C; Lankmayr, E; Spener, F.
A comprehensive method for lipid profiling by liquid chromatography-ion cyclotron resonance mass spectrometry.
J Lipid Res. 2011; 52(12):2314-2322
Doi: 10.1194/jlr.D016550
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- Führende Autor*innen der Med Uni Graz
- Köfeler Harald
- Trötzmüller Martin
- Co-Autor*innen der Med Uni Graz
- Hartler Jürgen
- Knopf Astrid
- Spener Friedrich
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- Abstract:
- This work aims to combine chromatographic retention, high mass resolution and accuracy, MS/MS spectra, and a package for automated identification and quantitation of lipid species in one platform for lipidomic analysis. The instrumental setup elaborated comprises reversed-phase HPLC coupled to a Fourier transform ion cyclotron resonance mass spectrometer (LTQ-FT), and Lipid Data Analyzer (LDA) software. Data analysis for lipid species quantification in this platform is based on retention time, mass resolution of 200,000, and mass accuracy below 2 ppm. In addition, automatically generated MS/MS spectra provide structural information at molecular level. This LC/MS technology allows analyzing complex biological samples in a quantitative manner as shown here paradigmatically for murine lipid droplets having a huge surplus of triacylglycerol species. Chromatographic preseparation of the bulk lipid class alleviates the problem of ion suppression of lipid species from other classes. Extension of 1D to 2D chromatography is possible, yet time consuming. The platform affords unambiguous detection of lipid species as low as 0.1 within major lipid classes. Taken together, a novel lipidomic LC/MS platform based on chromatographic retention, high mass resolution and accuracy, MS/MS analysis, and quantitation software enables analysis of complex samples as demonstrated for lipid droplets.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Chromatography, High Pressure Liquid - methods
- Chromatography, Reverse-Phase -
- Cyclotrons -
- Lipids - analysis
- Mice -
- Mice, Inbred C57BL -
- Reproducibility of Results -
- Tandem Mass Spectrometry - instrumentation
- Time Factors -
- Find related publications in this database (Keywords)
- high-resolution mass spectrometry
- lipidomics
- HPLC/ESI-MS
- HILIC
- lipid droplets
- triacylglycerols
- phospholipids