Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Navigation/Suche
- Forscher*innen
- Institutionen
- Projekte
- Publikationen
- Partner
- Geldgeber
- Ausstattung
- Knowhow
- Forschungsfelder
"Köpfe" der aktuellen Meldungen:
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Ritsch, A; Tancevski, I; Schgoer, W; Pfeifhofer, C; Gander, R; Eller, P; Foeger, B; Stanzl, U; Patsch, JR.
Molecular characterization of rabbit scavenger receptor class B types I and II: portal to central vein gradient of expression in the liver.
J Lipid Res. 2004; 45(2): 214-222.
Doi: 10.1194/jlr.M300353-JLR200
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Eller Philipp
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- To further elucidate the role of scavenger receptor class B type I (SR-BI) in reverse cholesterol transport and in atherogenesis, we performed studies in the rabbit, an animal model displaying a lipoprotein profile similar to that of human, expressing cholesteryl ester transfer protein in plasma and having been demonstrated to be susceptible to atherosclerosis. In this report, we describe for the first time the isolation and characterization of rabbit cDNA fragments encoding SR-BI and scavenger receptor class B type II (SR-BII). Development of an isoform-specific Taqman Real Time PCR system and generation of isoform-specific polyclonal antibodies allowed us to measure SR-BI and SR-BII expression in various rabbit organs on mRNA and protein levels, respectively. We found the highest expression of SR-BI in adrenal gland, liver, and proximal intestine; lesser expression was found in appendix and spleen. Immunohistochemical staining of frozen sections showed SR-BI expression in the cortex but not in the medulla of adrenal gland. An increasing portal to central vein gradient of expression was found within the hepatic lobule. As shown in this report, identification and characterization of SR-BI expression in the rabbit affords a powerful tool to elucidate the role of SR-BI in cholesterol homeostasis and atherogenesis in human.
- Find related publications in this database (using NLM MeSH Indexing)
- Adrenal Cortex - cytology Adrenal Cortex - metabolism
- Amino Acid Sequence -
- Animals -
- Arteriosclerosis - metabolism
- Biological Transport - genetics
- Cattle -
- Cholesterol - metabolism
- Cloning, Molecular -
- Cricetinae -
- Gene Expression - physiology
- Hepatic Veins - metabolism
- Humans -
- Liver - blood supply Liver - cytology Liver - metabolism
- Lysosome-Associated Membrane Glycoproteins -
- Membrane Proteins -
- Mice -
- Molecular Sequence Data -
- Rabbits -
- Rats -
- Receptors, Immunologic - biosynthesis Receptors, Immunologic - genetics
- Receptors, Scavenger -
- Sequence Alignment -
- Sialoglycoproteins -
- Spleen - cytology Spleen - metabolism
- Find related publications in this database (Keywords)
- antibody
- atherosclerosis
- cDNA cloning
- enzyme-linked immunosorbent assay
- high density lipoprotein
- immunohistochemical staining
- real-time polymerase chain reaction
- reverse cholesterol transport