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Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Sturm, EM; Schuligoi, R; Konya, V; Sturm, GJ; Heinemann, A.
Inhibitory effect of prostaglandin I2 on bone marrow kinetics of eosinophils in the guinea pig.
J Leukoc Biol. 2011; 90(2):285-291
Doi: 10.1189/jlb.0211087
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- Führende Autor*innen der Med Uni Graz
- Böhm Eva
- Co-Autor*innen der Med Uni Graz
- Heinemann Akos
- Konya Viktoria
- Schuligoi Rufina
- Sturm Gunter
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- Abstract:
- Enhanced eosinophil trafficking from bone marrow to the tissue is a hallmark of allergic diseases. We have shown previously that PGI(2) markedly attenuates the locomotion of human eosinophils in vitro. Here, we set out to determine the effect of PGI(2) on the trafficking of bone marrow eosinophils in the guinea pig. Shape change of bone marrow eosinophils was determined by flow cytometry, and chemotaxis assays were performed using a transwell migration system. Eosinophil release from bone marrow of guinea pigs was investigated in the isolated, perfused hind-limb preparation. We found that PGI(2) prevented the mobilization of eosinophils from bone marrow and attenuated the shape change and chemotactic responses of bone marrow eosinophils. These effects were mimicked by iloprost and were prevented by the IP antagonist CAY10441 and the adenylyl cyclase inhibitor SQ22536. Immunohistochemical staining of bone marrow confirmed the expression of IPs by bone marrow eosinophils. The rate-limiting enzyme of PGI(2) formation, PGIS, was found in large mononuclear cells. These data show that IP activation negatively modulates the mobilization and locomotion of bone marrow eosinophils and might therefore also protect against exaggerated recruitment of eosinophils to inflammatory sites.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Bone Marrow Cells -
- Cell Movement - drug effects
- Cell Shape - drug effects
- Cytochrome P-450 Enzyme System - analysis
- Eosinophils - cytology
- Epoprostenol - pharmacology
- Extremities -
- Guinea Pigs -
- Iloprost - pharmacology
- Intramolecular Oxidoreductases - analysis
- Kinetics -
- Find related publications in this database (Keywords)
- IP receptor
- PGI synthase
- trafficking