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SHR Neuro Krebs Kardio Lipid Stoffw Microb

Bondarenko, OI; Sahach, VF.
Modulation of electrical responses of endothelial cells by nicotinic cholinoreceptors].
Fiziol Zh. 2009; 55(2):17-23
PubMed

 

Führende Autor*innen der Med Uni Graz
Bondarenko Oleksandr
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Abstract:
Because the sustained component of hyperpolarization of endothelial cells evoked by acetylcholine in isolated rat aorta may partially be mediated by the reversed Na(+)-Ca(2+) exchanger and Na(+)-K(+) ATPase, the mechanisms which transport Na(+) out of cells, we compared the electrical responses of endothelial cells from isolated rat aorta to acetylcholine with other Ca(2+) mobilizing agents and studied the effect of nicotine on endothelial membrane potential in order to asses the functional activity of nicotinic cholinoreceptors. Ca(2+) ionophores A23187 and ionomycin, as well as inhibitors of endoplasmic reticulum Ca(2+) ATPase cyclopiazonic acid and 2,5-di-tert-butylhydroquinone evoked a short-lived hyperpolarization which turned to a sustained depolarization of endothelial cells, a time course that substantially differed from that evoked by acetylcholine. Nicotine evoked a Na+ dependent depolarization of endothelial cells confirming functional activity ofnicotinic cholinoreceptors in rat aortic endothelial cells. The results suggest that stimulation of Na+ permeant nicotinic receptors by acetylcholine may contribute to sustained hyperpolarizatiom via stimulation of Na+ extrusion mechanisms.
Find related publications in this database (using NLM MeSH Indexing)
Acetylcholine - pharmacology
Animals -
Aorta, Thoracic - drug effects Aorta, Thoracic - metabolism Aorta, Thoracic - physiology
Endoplasmic Reticulum - drug effects Endoplasmic Reticulum - metabolism Endoplasmic Reticulum - physiology
Endothelial Cells - drug effects Endothelial Cells - metabolism Endothelial Cells - physiology
Endothelium, Vascular - drug effects Endothelium, Vascular - metabolism Endothelium, Vascular - physiology
In Vitro Techniques -
Membrane Potentials - drug effects
Nicotine - pharmacology
Patch-Clamp Techniques -
Rats -
Receptors, Nicotinic - metabolism Receptors, Nicotinic - physiology
Sodium-Calcium Exchanger - metabolism
Sodium-Potassium-Exchanging ATPase - metabolism

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