Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Wittmann, L; Eigenfeld, M; Büchner, K; Meiler, J; Habisch, H; Madl, T; Kerpes, R; Becker, T; Berensmeier, S; Schwaminger, SP.
Millifluidic magnetophoresis-based chip for age-specific fractionation: evaluating the impact of age on metabolomics and gene expression in yeast.
Lab Chip. 2024; 24(11):2987-2998
Doi: 10.1039/d4lc00185k
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- Führende Autor*innen der Med Uni Graz
- Schwaminger Sebastian
- Co-Autor*innen der Med Uni Graz
- Becker Tatjana
- Eigenfeld Marco
- Habisch Hansjörg
- Madl Tobias
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- Abstract:
- A novel millifluidic process introduces age-based fractionation of S. pastorianus var. carlsbergensis yeast culture through magnetophoresis. Saccharomyces yeast is a model organism for aging research used in various industries. Traditional age-based cell separation methods were labor-intensive, but techniques like magnetic labeling have eased the process by being non-invasive and scalable. Our approach introduces an age-specific fractionation using a 3D-printed millfluidic chip in a two-step process, ensuring efficient cell deflection in the magnetic field and counteracting magnetic induced convection. Among various channel designs, the pinch-shaped channel proved most effective for age differentiation based on magnetically labeled bud scar numbers. Metabolomic analyses revealed changes in certain amino acids and increased NAD+ levels, suggesting metabolic shifts in aging cells. Gene expression studies further underlined these age-related metabolic changes. This innovative platform offers a high-throughput, non-invasive method for age-specific yeast cell fractionation, with potential applications in industries ranging from food and beverages to pharmaceuticals.
- Find related publications in this database (using NLM MeSH Indexing)
- Metabolomics - administration & dosage
- Saccharomyces - metabolism
- Microfluidic Analytical Techniques - instrumentation
- Saccharomyces cerevisiae - metabolism
- Lab-On-A-Chip Devices - administration & dosage