Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Wölflingseder, M; Fengler, VH; Standhartinger, V; Wagner, GE; Reidl, J.
The regulatory network comprising ArcAB-RpoS-RssB influences motility in Vibrio cholerae.
Mol Microbiol. 2024; 121(5):850-864
Doi: 10.1111/mmi.15235
Web of Science
PubMed
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FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Fengler Vera
- Wagner-Lichtenegger Gabriel
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- Abstract:
- The diarrheal disease cholera is caused by the versatile and responsive bacterium Vibrio cholerae, which is capable of adapting to environmental changes. Among others, the alternative sigma factor RpoS activates response pathways, including regulation of motility- and chemotaxis-related genes under nutrient-poor conditions in V. cholerae. Although RpoS has been well characterised, links between RpoS and other regulatory networks remain unclear. In this study, we identified the ArcAB two-component system to control rpoS transcription and RpoS protein stability in V. cholerae. In a manner similar to that seen in Escherichia coli, the ArcB kinase not only activates the response regulator ArcA but also RssB, the anti-sigma factor of RpoS. Our results demonstrated that, in V. cholerae, RssB is phosphorylated by ArcB, which subsequently activates RpoS proteolysis. Furthermore, ArcA acts as a repressor of rpoS transcription. Additionally, we determined that the cysteine residue at position 180 of ArcB is crucial for signal recognition and activity. Thus, our findings provide evidence linking RpoS response to the anoxic redox control system ArcAB in V. cholerae.
- Find related publications in this database (using NLM MeSH Indexing)
- Bacterial Proteins - metabolism, genetics
- Chemotaxis - genetics
- DNA-Binding Proteins - metabolism, genetics
- Gene Expression Regulation, Bacterial - administration & dosage
- Gene Regulatory Networks - administration & dosage
- Phosphorylation - administration & dosage
- Repressor Proteins - metabolism, genetics
- Sigma Factor - metabolism, genetics
- Transcription Factors - metabolism, genetics
- Vibrio cholerae - genetics, metabolism
- Find related publications in this database (Keywords)
- ArcA
- ArcB
- motility
- RpoS
- RssB
- Vibrio cholerae