Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Freise, L; Behncke, RY; Allerkamp, HH; Sandermann, TH; Chu, NH; Funk, EM; Hondrich, LJ; Riedel, A; Witzel, C; Hansmeier, NR; Danyel, M; Gellhaus, A; Dechend, R; Hägerling, R.
Three-Dimensional Histological Characterization of the Placental Vasculature Using Light Sheet Microscopy.
Biomolecules. 2023; 13(6):
Doi: 10.3390/biom13061009
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- Co-Autor*innen der Med Uni Graz
- Allerkamp Hanna
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- Abstract:
- The placenta is the first embryonic organ, representing the connection between the embryo and the mother, and is therefore necessary for the embryo's growth and survival. To meet the ever-growing need for nutrient and gas exchange, the maternal spiral arteries undergo extensive remodeling, thus increasing the uteroplacental blood flow by 16-fold. However, the insufficient remodeling of the spiral arteries can lead to severe pregnancy-associated disorders, including but not limited to pre-eclampsia. Insufficient endovascular trophoblast invasion plays a key role in the manifestation of pre-eclampsia; however, the underlying processes are complex and still unknown. Classical histopathology is based on two-dimensional section microscopy, which lacks a volumetric representation of the vascular remodeling process. To further characterize the uteroplacental vascularization, a detailed, non-destructive, and subcellular visualization is beneficial. In this study, we use light sheet microscopy for optical sectioning, thus establishing a method to obtain a three-dimensional visualization of the vascular system in the placenta. By introducing a volumetric visualization method of the placenta, we could establish a powerful tool to deeply investigate the heterogeneity of the spiral arteries during the remodeling process, evaluate the state-of-the-art treatment options, effects on vascularization, and, ultimately, reveal new insights into the underlying pathology of pre-eclampsia.
- Find related publications in this database (using NLM MeSH Indexing)
- Humans - administration & dosage
- Pregnancy - administration & dosage
- Female - administration & dosage
- Placenta - blood supply
- Pre-Eclampsia - pathology
- Microscopy - administration & dosage
- Trophoblasts - pathology
- Arteries - pathology
- Pregnancy Complications - administration & dosage
- Find related publications in this database (Keywords)
- light sheet microscopy
- placenta
- histopathology
- lymphatic mimicry
- pre-eclampsia