Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Kotzbeck, P; Taschler, U; Haudum, C; Foessl, I; Schoiswohl, G; Boulgaropoulos, B; Bounab, K; Einsiedler, J; Pajed, L; Tilp, A; Schwarz, A; Eichmann, TO; Obermayer-Pietsch, B; Giordano, A; Cinti, S; Zechner, R; Pieber, TR.
Rosiglitazone Reverses Inflammation in Epididymal White Adipose Tissue in Hormone-Sensitive Lipase-Knockout Mice.
J Lipid Res. 2023; 64(1):100305
Doi: 10.1016/j.jlr.2022.100305
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- Führende Autor*innen der Med Uni Graz
- Kotzbeck Petra
- Co-Autor*innen der Med Uni Graz
- Boulgaropoulos Beate
- Bounab Kaddour
- Eichmann Thomas
- Fößl Ines
- Haudum Christoph
- Obermayer-Pietsch Barbara
- Pieber Thomas
- Schoiswohl Gabriele Maria
- Schwarz Anna
- Taschler Ulrike
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- Abstract:
- Hormone-sensitive lipase (HSL) plays a crucial role in intracellular lipolysis, and loss of HSL leads to diacylglycerol (DAG) accumulation, reduced FA mobilization, and impaired PPARγ signaling. Hsl knockout mice exhibit adipose tissue inflammation, but the underlying mechanisms are still not clear. Here, we investigated if and to what extent HSL loss contributes to endoplasmic reticulum (ER) stress and adipose tissue inflammation in Hsl knockout mice. Furthermore, we were interested in how impaired PPARγ signaling affects the development of inflammation in epididymal white adipose tissue (eWAT) and inguinal white adipose tissue (iWAT) of Hsl knockout mice and if DAG and ceramide accumulation contribute to adipose tissue inflammation and ER stress. Ultrastructural analysis showed a markedly dilated ER in both eWAT and iWAT upon loss of HSL. In addition, Hsl knockout mice exhibited macrophage infiltration and increased F4/80 mRNA expression, a marker of macrophage activation, in eWAT, but not in iWAT. We show that treatment with rosiglitazone, a PPARγ agonist, attenuated macrophage infiltration and ameliorated inflammation of eWAT, but expression of ER stress markers remained unchanged, as did DAG and ceramide levels in eWAT. Taken together, we show that HSL loss promoted ER stress in both eWAT and iWAT of Hsl knockout mice, but inflammation and macrophage infiltration occurred mainly in eWAT. Also, PPARγ activation reversed inflammation but not ER stress and DAG accumulation. These data indicate that neither reduction of DAG levels nor ER stress contribute to the reversal of eWAT inflammation in Hsl knockout mice.
- Find related publications in this database (using NLM MeSH Indexing)
- Mice - administration & dosage
- Animals - administration & dosage
- Rosiglitazone - pharmacology
- Sterol Esterase - genetics, metabolism
- Mice, Knockout - administration & dosage
- PPAR gamma - genetics, metabolism
- Adipose Tissue - metabolism
- Adipose Tissue, White - metabolism
- Lipolysis - physiology
- Inflammation - drug therapy, genetics, metabolism
- Find related publications in this database (Keywords)
- adipocytes
- adipose tissue
- lipolysis
- FA metabolism
- lipase
- inflammation
- dysfunctional adipocytes
- FA
- electron microscopy
- lipotoxicity