Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Dichtl, K; Osterman, A; Barry, R; Wagener, J.
A novel microarray-based PCR assay for the detection of HSV-1, HSV-2, and VZV skin infections: A retrospective analysis.
J Virol Methods. 2023; 312:114650
Doi: 10.1016/j.jviromet.2022.114650
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Dichtl Karl
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- Abstract:
- Prevalence of HSV-1, HSV-2, and VZV infection ranges from 20% to 90%. Viral reactivation is common and results in a significant individual and socioeconomic burden. Pathognomonic skin manifestations are not always present, impairing definitive clinical diagnosis. We evaluated the performance of a novel microarray-based multiplex PCR system (Euroarray, Euroimmun Medizinische Labordiagnostika) for the molecular detection of these pathogens. In this retrospective study, 50 consecutive specimens positive for HSV-1, HSV-2, or VZV (pre-characterized by qPCR) were analyzed. Two hundred-and-five negative test results were applied as a control group. The microarray successfully detected the respective pathogens in all samples that yielded a qPCR quantifiable amount of DNA. Two and one specimens containing VZV and HSV-1 DNA beneath the limit of quantification tested microarray negative. Microarray specificity was 100%. The microarray is a useful tool for diagnosing viral infections of skin and mucous membranes, allowing rapid differentiation between three pathogens in a single assay.
- Find related publications in this database (using NLM MeSH Indexing)
- Humans - administration & dosage
- Herpesvirus 1, Human - genetics
- Herpesvirus 2, Human - genetics
- Retrospective Studies - administration & dosage
- Herpes Simplex - diagnosis
- Herpesvirus 3, Human - genetics
- DNA, Viral - genetics, analysis
- Sensitivity and Specificity - administration & dosage
- Multiplex Polymerase Chain Reaction - methods
- Find related publications in this database (Keywords)
- Skin infection
- Herpes
- HSV-1
- HSV-2
- VZV
- PCR