Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Gindlhuber, J; Schinagl, M; Liesinger, L; Darnhofer, B; Tomin, T; Schittmayer, M; Birner-Gruenberger, R.
Hepatocyte Proteome Alterations Induced by Individual and Combinations of Common Free Fatty Acids.
Int J Mol Sci. 2022; 23(6):
Doi: 10.3390/ijms23063356
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- Führende Autor*innen der Med Uni Graz
- Birner-Grünberger Ruth
- Gindlhuber Jürgen
- Co-Autor*innen der Med Uni Graz
- Darnhofer Barbara
- Liesinger Laura
- Schinagl Maximilian
- Schittmayer-Schantl Matthias
- Tomin Tamara
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- Abstract:
- Non-alcoholic fatty liver disease is a pathology with a hard-to-detect onset and is estimated to be present in a quarter of the adult human population. To improve our understanding of the development of non-alcoholic fatty liver disease, we treated a human hepatoma cell line model, HepG2, with increasing concentrations of common fatty acids, namely myristic, palmitic and oleic acid. To reproduce more physiologically representative conditions, we also included combinations of these fatty acids and monitored the cellular response with an in-depth proteomics approach and imaging techniques. The two saturated fatty acids initially presented a similar phenotype of a dose-dependent decrease in growth rates and impaired lipid droplet formation. Detailed analysis revealed that the drop in the growth rates was due to delayed cell-cycle progression following myristic acid treatment, whereas palmitic acid led to cellular apoptosis. In contrast, oleic acid, as well as saturated fatty acid mixtures with oleic acid, led to a dose-dependent increase in lipid droplet volume without adverse impacts on cell growth. Comparing the effects of harmful single-fatty-acid treatments and the well-tolerated fatty acid mixes on the cellular proteome, we were able to differentiate between fatty-acid-specific cellular responses and likely common lipotoxic denominators.
- Find related publications in this database (using NLM MeSH Indexing)
- Fatty Acids - metabolism
- Fatty Acids, Nonesterified - metabolism, pharmacology
- Hepatocytes - metabolism
- Humans - administration & dosage
- Non-alcoholic Fatty Liver Disease - metabolism
- Oleic Acid - metabolism, pharmacology
- Palmitic Acid - metabolism, pharmacology
- Proteome - metabolism
- Find related publications in this database (Keywords)
- NAFLD
- lipotoxicity
- myristic acid
- palmitic acid
- oleic acid