Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Runtsch, MC; Angiari, S; Hooftman, A; Wadhwa, R; Zhang, Y; Zheng, Y; Spina, JS; Ruzek, MC; Argiriadi, MA; McGettrick, AF; Mendez, RS; Zotta, A; Peace, CG; Walsh, A; Chirillo, R; Hams, E; Fallon, PG; Jayamaran, R; Dua, K; Brown, AC; Kim, RY; Horvat, JC; Hansbro, PM; Wang, C; O'Neill, LAJ.
Itaconate and itaconate derivatives target JAK1 to suppress alternative activation of macrophages.
Cell Metab. 2022; 34(3): 487-501.e8.
Doi: 10.1016/j.cmet.2022.02.002
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Runtsch Marah
- Co-Autor*innen der Med Uni Graz
- Angiari Stefano
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- Abstract:
- The Krebs cycle-derived metabolite itaconate and its derivatives suppress the inflammatory response in pro-inflammatory "M1" macrophages. However, alternatively activated "M2" macrophages can take up itaconate. We therefore examined the effect of itaconate and 4-octyl itaconate (OI) on M2 macrophage activation. We demonstrate that itaconate and OI inhibit M2 polarization and metabolic remodeling. Examination of IL-4 signaling revealed inhibition of JAK1 and STAT6 phosphorylation by both itaconate and OI. JAK1 activation was also inhibited by OI in response to IL-13, interferon-β, and interferon-γ in macrophages and in T helper 2 (Th2) cells. Importantly, JAK1 was directly modified by itaconate derivatives at multiple residues, including cysteines 715, 816, 943, and 1130. Itaconate and OI also inhibited JAK1 kinase activity. Finally, OI treatment suppressed M2 macrophage polarization and JAK1 phosphorylation in vivo. We therefore identify itaconate and OI as JAK1 inhibitors, suggesting a new strategy to inhibit JAK1 in M2 macrophage-driven diseases.