Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Zarin, T; Strome, B; Peng, G; Pritišanac, I; Forman-Kay, JD; Moses, AM.
Identifying molecular features that are associated with biological function of intrinsically disordered protein regions.
Elife. 2021; 10:
Doi: 10.7554/eLife.60220
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Pritisanac Iva
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- In previous work, we showed that intrinsically disordered regions (IDRs) of proteins contain sequence-distributed molecular features that are conserved over evolution, despite little sequence similarity that can be detected in alignments (Zarin et al., 2019). Here, we aim to use these molecular features to predict specific biological functions for individual IDRs and identify the molecular features within them that are associated with these functions. We find that the predictable functions are diverse. Examining the associated molecular features, we note some that are consistent with previous reports and identify others that were previously unknown. We experimentally confirm that elevated isoelectric point and hydrophobicity, features that are positively associated with mitochondrial localization, are necessary for mitochondrial targeting function. Remarkably, increasing isoelectric point in a synthetic IDR restores weak mitochondrial targeting. We believe feature analysis represents a new systematic approach to understand how biological functions of IDRs are specified by their protein sequences.
- Find related publications in this database (using NLM MeSH Indexing)
- Amino Acid Sequence - administration & dosage
- Hydrophobic and Hydrophilic Interactions - administration & dosage
- Intrinsically Disordered Proteins - chemistry, metabolism
- Isoelectric Point - administration & dosage
- Mitochondria - metabolism
- Models, Statistical - administration & dosage
- Proteome - chemistry, metabolism
- Saccharomyces cerevisiae - metabolism