Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Greimel, TM; Stampfer, L; Leitner, E; Kienesberger, S; Zechner, EL; Bozic, M; Wagner, GE; Unterhauser, K; Kitsera, M; Hauer, AC; Gorkiewicz, G; Wurm, P; Valitutti, F; Högenauer, C; Hoffmann, KM.
Toxin-Producing Klebsiella oxytoca in Healthy Infants: Commensal or Pathobiont?
J Pediatr Gastroenterol Nutr. 2022; 74(1):e1-e7
Doi: 10.1097/MPG.0000000000003299
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Bauer Theresa Margarete
- Hoffmann Karl Martin
- Co-Autor*innen der Med Uni Graz
- Bozic Michael
- Gorkiewicz Gregor
- Hauer Almuthe
- Hoegenauer Christoph
- Kienesberger-Feist Sabine
- Leitner-Meyer Eva
- Stampfer Laura
- Wagner-Lichtenegger Gabriel
- Wurm Philipp
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- OBJECTIVES: Klebsiella oxytoca is a gastrointestinal pathobiont with the potential to produce the toxins tilivalline and tilimycin, which cause antibiotic-associated hemorrhagic colitis. Overgrowth of toxigenic K oxytoca has recently been implicated in necrotizing enterocolitis. K oxytoca colonizes 2-9% of healthy adults, however, there is no systematic data on colonization in healthy children. We investigated K oxytoca colonization and its toxigenic properties in healthy infants. METHODS: We sampled stool of healthy infants and determined K oxytoca colonization using stool culture and PCR (pehX). Toxin in stool was measured with HPLC/high-resolution mass spectrometry. K oxytoca isolates were typed using multi-locus sequence typing (MLST) and K oxytoca toxin PCR (npsA/B). Cytotoxin production of isolates was analyzed by MTT assay. RESULTS: K oxytoca was detected in 30 of 61 infants (49%) using stool culture and in 45 of 61 (73%) using PCR (pehX). Toxin marker PCR (npsA/B) was positive in 66% of stool samples positive for K oxytoca PCR. Stool toxin levels were too low for quantitation but traces of tilivalline were detected. Contrarily, 49% of K oxytoca isolates demonstrated toxicity in the MTT assay. MLST revealed 36 distinct sequence types affiliated with all known K oxytoca sequence type clusters (A, B1 and B2). CONCLUSIONS: More than 70% of healthy infants were colonized with K oxytoca. Toxin quantities in stool of colonized healthy infants were below detection level, yet half of the isolates produced toxin in vitro demonstrating their pathobiont potential. The high occurrence of toxigenic K oxytoca in healthy infants has to be considered for future disease association studies.
- Find related publications in this database (Keywords)
- bacteria
- cytotoxins
- infants
- Klebsiella oxytoca
- microbiome
- pathobiont
- pediatrics