Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Gruber, M; Weiss, E; Siwetz, M; Hiden, U; Gauster, M.
Flow-through isolation of human first trimester umbilical cord endothelial cells.
Histochem Cell Biol. 2021; 156(4): 363-375.
Doi: 10.1007/s00418-021-02007-7
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- Führende Autor*innen der Med Uni Graz
- Gruber Michael
- Hiden Ursula
- Weiß Elisa
- Co-Autor*innen der Med Uni Graz
- Gauster Martin
- Siwetz Monika
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- Abstract:
- Human umbilical vein and artery endothelial cells (HUVEC; HUAEC), placental endothelial cells (fpAEC), and endothelial colony-forming cells (ECFC) from cord blood are a widely used model for researching placental vascular development, fetal and placental endothelial function, and the effect of adverse conditions in pregnancy thereon. However, placental vascular development and angiogenesis start in the first weeks of gestation, and adverse conditions in pregnancy may also affect endothelial function before term, suggesting that endothelial cells from early pregnancy may respond differently. Thus, we established a novel, gentle flow-through method to isolate pure human umbilical endothelial cells from first trimester (FTUEC). FTUEC were characterized and their phenotype was compared to the umbilical endothelium in situ as well as to other fetal endothelial cell models from term of gestation, i.e. HUVEC, fpAEC, ECFC. FTUEC possess a CD34-positive, juvenile endothelial phenotype, and can be expanded and passaged. We regard FTUEC as a valuable tool to study developmental processes as well as the effect of adverse insults in pregnancy in vitro.
- Find related publications in this database (Keywords)
- First trimester
- Umbilical cord
- Human placenta
- Endothelial cells
- Isolation
- HUVEC
- HUAEC