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SHR Neuro Cancer Cardio Lipid Metab Microb
Földes-Papp, Z; Liao, SC; Barbieri, B; Gryczynski, K; Luchowski, R; Gryczynski, Z; Gryczynski, I; Borejdo, J; You, T.
Single actomyosin motor interactions in skeletal muscle.
Biochim Biophys Acta. 2011; 1813(5):858-866
Doi: 10.1016/j.bbamcr.2011.02.001
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- Leading authors Med Uni Graz
- Földes-Papp Zeno
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- Abstract:
- We present a study of intramuscular motion during contraction of skeletal muscle myofibrils. Myofibrillar actin was labeled with fluorescent dye so that the ratio of fluorescently labeled to unlabeled protein was 1:10(5). Such sparse labeling assured that there was on average only one actin-marker present in the focus at a given time. From the intensity signal in the two orthogonal detection channels, significant fluctuations, similar to fluorescent burst in diffusion-based single-molecule detection schemes, were identified via a threshold algorithm and analyzed with respect to their intensity and polarization. When only rigor complexes were formed, the fluctuations of polarized intensity were characterized by unimodal Gaussian photon distributions. During contraction, in contrast, bimodal Gaussian photon distributions were observed above the rigor background threshold. This suggests that the bimodal Gaussian photon distributions represent pre- and post-power stroke conformations. Clusters of polarized photons indicated an anisotropy decay of single actomyosin motors of ~9s during muscle contraction. This article is part of a Special Issue entitled: 11th European Symposium on Calcium.
- Find related publications in this database (using NLM MeSH Indexing)
- Actomyosin - metabolism
- Animals -
- Fluorescence Polarization -
- Fluorescent Dyes - metabolism
- Molecular Motor Proteins - metabolism
- Muscle Contraction - physiology
- Muscle Rigidity - physiopathology
- Muscle, Skeletal - metabolism
- Protein Binding -
- Rabbits -
- Time Factors -
- Find related publications in this database (Keywords)
- Single actomyosin
- Live skeletal muscle cell
- Polarization dependent fluorescence fluctuations
- Time-dependent photon distributions
- Molecular orientations