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Selected Publication:
Pieske, B; Sütterlin, M; Schmidt-Schweda, S; Minami, K; Meyer, M; Olschewski, M; Holubarsch, C; Just, H; Hasenfuss, G.
Diminished post-rest potentiation of contractile force in human dilated cardiomyopathy. Functional evidence for alterations in intracellular Ca2+ handling.
J Clin Invest. 1996; 98(3):764-776
Doi: 10.1172/JCI118849
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- Leading authors Med Uni Graz
- Pieske Burkert Mathias
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- Abstract:
- Post-rest contractile behavior of isolated myocardium indicates the capacity of the sarcoplasmic reticulum (SR) to store and release Ca2+. We investigated post-rest behavior in isolated muscle strips from nonfailing (NF) and endstage failing (dilated cardiomyopathy [DCM]) human hearts. At a basal stimulation frequency of 1 Hz, contractile parameters of the first twitch after increasing rest intervals (2-240 s) were evaluated. In NF (n = 9), steady state twitch tension was 13.7 +/- 1.8 mN/mm2. With increasing rest intervals, post-rest twitch tension continuously increased to maximally 29.9 +/- 4.1 mN/mm2 after 120s (P < 0.05) and to 26.7 +/- 4.5 mN after 240 s rest. In DCM (n = 22), basal twitch tension was 10.0 +/- 1.5 mN/mm2 and increased to maximally 13.6 +/- 2.2 mN/mm2 after 20 s rest (P < 0.05). With longer rest intervals, however, post-rest twitch tension continuously declined (rest decay) to 4.7 +/- 1.0 mN/mm2 at 240 s (P < 0.05). The rest-dependent changes in twitch tension were associated with parallel changes in intracellular Ca2- transients in NF and DCM (aequorin method). The relation between rest-induced changes in twitch tension and aequorin light emission was similar in NF and DCM, indicating preserved Ca(2-)-responsiveness of the myofilaments. Ryanodine (1 microM) completely abolished post-rest potentiation. Increasing basal stimulation frequency (2 Hz) augmented post-rest potentiation, but did not prevent rest decay after longer rest intervals in DCM. The altered post-rest behavior in failing human myocardium indicates disturbed intracellular Ca2- handling involving altered function of the SR.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Aged -
- Calcium - metabolism
- Cardiomyopathy, Dilated - physiopathology
- Female - physiopathology
- Humans - physiopathology
- Male - physiopathology
- Middle Aged - physiopathology
- Myocardial Contraction - physiopathology
- Ryanodine - pharmacology
- Sarcoplasmic Reticulum - metabolism
- Stroke Volume - metabolism
- Find related publications in this database (Keywords)
- myocardial function
- excitation-contraction-coupling
- sarcoplasmic reticulum
- aequorin
- calcium handling