Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Foessl, I; Birngruber, T; Kotzbeck, P; Hofmann, E.
Ex Vivo Human Skin Model to Study Immediate and Early Local Responses to External Stimuli.
Methods Mol Biol. 2025; 2922:289-295
Doi: 10.1007/978-1-0716-4510-9_22
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Fößl Ines
- Co-Autor*innen der Med Uni Graz
- Birngruber Thomas
- Hofmann Elisabeth
- Kotzbeck Petra
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Local responses of skin tissue to external stimuli are complex, thus exceeding the capability of in vitro methods but do not need the complexity of a whole organism to be simulated. Therefore, we established a human ex vivo skin model in our lab to study the local effects of various stimuli, such as burns and local topic treatments. In this chapter, we describe the experimental setup of the ex vivo model and additional technology of collecting dermal interstitial fluid. Further, we describe how exosome-like vesicles can be enriched from the dermal interstitial fluid, including their proof via nanoparticle tracking.
- Find related publications in this database (using NLM MeSH Indexing)
- Humans - administration & dosage
- Skin - metabolism
- Extracellular Fluid - metabolism
- Exosomes - metabolism
- Nanoparticles - administration & dosage
- Models, Biological - administration & dosage