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Krolitzki, E; Winkler, W; Schwaminger, S; Berensmeier, S.
Bare magnetic iron oxides for binding and selective elution of lactoferrin from acid whey
COLLOID SURFACE A. 2025; 716: 136654 Doi: 10.1016/j.colsurfa.2025.136654
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Co-Autor*innen der Med Uni Graz: Schwaminger Sebastian
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Abstract:: Bare iron oxide nanoparticles (BIONs) can isolate the dilute but valuable protein lactoferrin (LF) from dairy byproducts through magnetic separation. While BIONs can be produced in large quantities at a reasonable price and can act as ion-exchanging material, the binding behaviors of biomolecules to BIONs in complex mixtures need to be better understood. Using a combination of static binding studies and zeta potential measurements, we investigated how whey salts and the whey proteins beta-lactoglobulin, bovine serum albumin, and alpha-lactalbumin influence the binding of LF to BIONs. Zeta potential measurements revealed that whey salts induce a surface charge reversal on BIONs, enabling them to function as cation exchangers at acidic pH. The cationic LF exhibited preferential binding to BIONs in single- and multicomponent binding experiments. Even in native acid whey, where LF accounted for less than 3 % of the whey protein, it constituted over 43 % of surface-bound whey protein on BIONs. By selectively controlling whey protein elution with simple salt buffers, we achieved complete LF elution with over 84 % purity. This study represents the first in-depth analysis of BIONs for the selective isolation of an untagged natural protein from a complex mixture, demonstrating their reusability over multiple cycles. Our findings enhance the understanding of bio-nano interactions and demonstrate the potential of magnetic separation with BIONs as a scalable and cost-effective solution for recovering valuable biomolecules from dustrial by-products.
Find related publications in this database (Keywords): Lactoferrin; Biocorona; Acid whey; Isolation; Downstream processing; Magnetic separation