Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Traunwieser, E.
Untersuchung der aseptischen Hüftprothesenlockerung unter Einfluss von Antioxidantien auf zellulärer Ebene.
[ Dissertation ] Medizinische Universität Graz; 2009. pp.75.
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- Autor*innen der Med Uni Graz:
- Wolf Elisabeth
- Betreuer*innen:
- Lohberger Birgit
- Schreibmayer Wolfgang
- Windhager Reinhard
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- Abstract:
- Introduction: The aseptic loosening is the main complication of implanted protheses after ten to twenty years. Wear debris from artificial joint surfaces is the limiting factor of the prosthesis lifetime. Due to the instability in artificial joints more synovial liquid and cysts appear which leads to an increase of cytokines. Depending on their size, shape and structure debris particles cause a biological answer of the immune-system with an increase of TNF-, IL1, IL6, PGR2, matrix-metalloproteinases, free radicals and other factors which can be quantified. New surface-materials like UHMW-PE which contains the antioxidant -Tocopherol and causes less debris are an attempt to reduce the incidence of aseptic prosthesis loosening. We investigated the ability of other antioxidants to decrease the biological answer to wear debris. Material and methods: In vitro experiments with human primary cells and mononuclear mouse macrophages (RAW293) were accomplished as followed: The macrophages and Primary cells were stimulated with LPS. The macrophages grade of activation was quantified by an increase of Peroxide compared to negative samples. In human primary cells the activation was measured by the increase of TNF- compared to negative samples. Antioxidants (Vitamin C, Quercetin, Resveratrol, NAC and -Tocopherol) were examined on their ability to decrease the oxidative stress levels after stimulation. Results: The human primary cells showed a highly significant increase of TNF- after stimulation with LPS while mouse-macrophages did not. The experiments with antioxidants showed that NAC reduced the oxidative Stress most effectively in both cell-lines. Quercetin induced a significant reduction in human primary cells and Resveratrol in mouse macrophages. Discussion: NAC and Resveratrol may play an important roll in designing new artificial joint materials and may reduce the incidence if aseptic joint loosening in the future.