Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Kristler, M.
Culture of smooth muscle cells for Tissue Engineering
[ Diplomarbeit/Master Thesis ] Graz Medical University; 2008. pp.55.
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- Autor*innen der Med Uni Graz:
- Betreuer*innen:
- Saxena Amulya Kumar
- Altmetrics:
- Abstract:
- Background: Management of congenital birth defects like short bowel syndrome, long-gap esophageal atresia, diaphragmatic hernia or dysplasia of the urinary tract poses challenges. Surgical techniques are associated with complications and high morbidity or are quite out of the question. Because smooth muscle cells (SMC) may act as an ubiquitary donor in the upper alluded defects the aim of this study was to develop protocols to obtain mono-layer sheets of SMC and to investigate their survival on collagen scaffolds. Methods: SMC were sourced from the aorta of adult Sprague Dawley rats. Surrounding tissue was removed by blunt dissection and the muscular tube was cut into small pieces and placed into Tissue Culture Treated Multiwell Plates. The SMC were proliferated and seeded on to a new generation of 3D collagen scaffolds. Results: SMC sprouted from the aortic pieces after 48-96 hours in static culture and after 14-20 days a 70-80% confluence was obtained in 24-Multiwell Plates. Immunohistochemistry stains demonstrated pure SMC culture using alpha-actin markers. The SMC seeded on to collagen polymers displayed deep infiltration, unidirectional growth along the tubular structures of the scaffold and excellent survivability of the cells after 8 weeks of static in-vitro culture. Conclusion: Successful protocols for SMC isolation and proliferation were established. The engineering of unidirectional SMC-strings and their survivability on collagen scaffolds for 8 weeks in-vitro, which are prerequisites for gastrointestinal or cardiovascular tissue engineering, was demonstrated.