Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Wagner, J.
Determination of HCV and HIV-1 RNA in whole saliva specimens
[ Dissertation ] Graz Medical University; 2007. pp.51.
[OPEN ACCESS]
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- Autor*innen der Med Uni Graz:
- Rabensteiner Jasmin
- Betreuer*innen:
- Haushofer Alexander
- Kessler Harald
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- Abstract:
- Background: Both hepatitis C virus (HCV) RNA and human immunodeficiency virus type 1 (HIV-1) RNA may be present in and transmitted through saliva. Methods: Whole saliva and blood samples were collected from 40 patients with chronic HCV infection without anti-HCV therapy and from 16 patients with HIV-1 infection. The Greiner Bio-One Saliva Collection System was used for standardized collection of whole saliva samples. Viral loads were determined by commercially available molecular assays. Results obtained from blood samples and corresponding whole saliva samples were compared. Results: Serum HCV RNA concentrations ranged from 2.7 x 10E4 to 4.4 x 10E7 IU/mL. Of the corresponding whole saliva samples, 14 samples were tested positive, 13 were found to be ULLD, and 13 showed an inhibition. Of the 14 positives, HCV RNA concentrations in saliva ranged from less than 15 to 4.9 x 10E2 IU/mL. Plasma HIV-1 RNA concentrations ranged from 7.4 x 10E2 to 4.1 x 10E4 copies/mL. Of the corresponding whole saliva samples, 2 were tested positive, 2 were found to be ULLD, and 12 showed an inhibition. Of the 2 positives, HIV-1 RNA concentrations in saliva were found to be 1.9 x 10E2 and 9.6 x 10E3 copies/mL. Conclusion: Although the infective dose remains unknown, exposure to contaminated saliva may be responsible for transmission of HCV and HIV-1.