Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Rebernig, HJ.
Identification of Maternal Cells in the Human Placenta.
[ Diplomarbeit/Master Thesis (UNI) ] Universität Graz; 2024. pp.107.
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- Autor*innen der Med Uni Graz:
- Betreuer*innen:
- Kroneis Thomas
- Altmetrics:
- Abstract:
- Microchimerism (MC) is defined as the persistent presence of low frequency fetal cells in the maternal body or vice versa. MC research has yielded conflicting results, with some studies advocating its potential benefits for human health and others highlighting its potential involvement in human pathology. The accurate detection of this low frequency MC cell population in another individual, however, poses a methodological challenge, vulnerable to potential biases. Therefore, the validation of obtained results, particularly in the scope of MC research, remains of crucial importance. The primary objectives of my thesis were, first to identify previously fluorescently labeled maternal cells in ex vivo perfused placental samples obtained from women with male pregnancies; and second to verify their maternal origin using an alternative methodology.Immunofluorescent (IF) staining for previously fluorescently labeled cells in ex vivo perfused placental samples and subsequent fluorescent imaging was performed. IF-stained cells were manually counted. Subsequently, XY fluorescence In Situ hybridization (FISH) on IF-treated placental samples was performed and the chromosomal composition of IF-stained counted cells was analyzed by fluorescence microscopy. Based on validation of fluorescent dye properties and IF staining specificity, a candidate maternal perfused cell population within ex vivo perfused placental samples according to IF could be identified. Subsequently, their maternal origin could be confirmed or refuted by XY-FISH. Despite this dual confirmatory approach of maternal identity, it is crucial to interpret the results with caution. This emphasizes a critical aspect of MC research, where result validation and transparency are frequently overlooked but indisputably vital.