Medizinische Universität Graz - Research portal
Selected Publication:
Lisic, A.
Investigating changes in mitochondrial metabolism during aging in nematodes and aging cells.
[ Diplomarbeit/Master Thesis (UNI) ] TU Graz; 2023. pp.73.
- Authors Med Uni Graz:
- Advisor:
- Madreiter-Sokolowski Corina
- Altmetrics:
- Abstract:
- Aging is an unavoidable process in almost all organisms, which is highly complex and not well understood. It is also an important risk factor for a variety of diseases. The most common age-related diseases include cardiovascular diseases, neurodegenerative diseases such as Alzheimer's and Parkinson's disease, and metabolic diseases such as atherosclerosis and diabetes. Therefore, it is crucial to understand molecular mechanisms of the aging process and to investigate therapeutic interventions that can prolong lifespan and improve health. In the current study, we investigated the metabolism during aging in nematodes and aging cells. Caenorhabditis elegans (C. elegans) strains N2 (wild type), CF1058 (daf16 deficiency), and EU31 (skn-1 deficiency) have been analyzed by NMR spectroscopy to identify metabolic features of aging and differences between the strains. SPLS-DA and O-PLS-DA revealed significant group separation between the age groups for the C. elegans strains. We also identified the most prominent ten alterations of metabolites during different stages of aging. Individual metabolite levels during aging revealed linear changes in the nematodes, like metabolites of essential amino acids. In contrast, metabolites of the TCA cycle or non-essential amino acids revealed biphasic alterations. (...)