Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Berner, A.
Incorporation of non-canonical amino acids into proteins of B. pseudomallei & Development and validation of a lateral flow assay for detection of Burkholderia mallei infection in horses.
[ Diplomarbeit/Master Thesis (UNI) ] Universität Graz; 2019. pp.60.
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- Autor*innen der Med Uni Graz:
- Betreuer*innen:
- Wagner-Lichtenegger Gabriel
- Altmetrics:
- Abstract:
- B. pseudomallei is the causative agent of melioidosis, an infectious and often fatal disease affecting around 165.000 people per year estimated mortality rate of 54%. Many mechanisms of the pathogen regarding infection, host evasion and resistance are not fully understood yet. Hence new tools for the investigations of the molecular basis of these mechanisms in B. pseudomallei are needed. A promising new way is the incorporation of non-canonical amino acids (ncAAs) into proteins. More than 150 different ncAAs have been introduced into proteins using the amber stop codon and a corresponding tRNA/aminoacyl-tRNA synthetase pair. These ncAAs, which are amino acids not used in nature to build proteins, can have fluorescent, cross-linking or redox-active functions. Their divers physical, chemical and biological properties have been exploited for a variety of biological studies and biotechnical applications already in many organisms including E. coli, yeast and mammalian cells. Therefore, providing a powerful tool for studying protein-protein interactions, host-pathogen interaction and many others. The plasmid created in this thesis is the first step to bring this powerful tool to B. pseudomallei research and to enable scientists to answer biological questions in a completely new way.