Medizinische Universität Graz - Research portal
Selected Publication:
Galistl, K.
Comparative in-vitro and in-vivo studies of PCUPLA-modifications and FISH skin as cell-seeded wound dressings.
[ Diplomarbeit/Master Thesis (UNI) ] TU Graz; 2024. pp.96.
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- Authors Med Uni Graz:
- Advisor:
- Lang-Olip Ingrid
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- Abstract:
- Already several studies demonstrated the advantageous effects of mesenchymal stromal cells (MSC) in the process of cutaneous wound healing. Biodegradable matrixes such as polycaprolactone/polylactide (PCL/PLA) and decellularized FISH skin seeded with MSC provide a promising skin replacement for clinically challenging wounds. The aim of this study was to compare 3 different materials, PCL/PLA-1, PCL/PLA-2, and FISH skin, for the suitability as carriers for MSC in in-vitro. Further, in an in-vivo mouse model the 3 carrier materials seeded with MSC were applied on dermal wounds and the abidance of the MSC within the wound tissue was examined. Adipose-tissue derived MSC (AD-MSC) obtained from 4 donors were tested for the angiogenic potential by coculturing the AD-MSC of each donor with endothelial cells (EC) for 8 days. Immunohistochemical (IHC) staining with anti-VE Cadherin showed that AD-MSC of donor 10 has the highest angiogenic potential as most endothelial networks have been formed. PCL/PLA carriers and the FISH skin carriers were seeded with a co-culture of AD-MSC or MSC derived from the amnion (AMSC) and EC and incubated for 7 or 14 days. Histological and IHC evaluation showed that PCL/PLA-1 and FISH skin material displayed an attachment of and infiltration by MSC and a high angiogenic potential of the MSC. PCL/PLA-2 could not be studied in in-vitro due to melting of the material during the paraffin embedding process. For the in-vivo study the carrier materials were seeded with AD-MSC or AMSC and cultured for 3 days in in-vitro before applying them on the mice wounds. Wound biopsy samples were taken 3, 7, 14 and 21 days after wounding and evaluated by histological and IHC methods. Interestingly, MSC in the present study were still detectable in wound biopsy samples 14 and 21 days post wounding whereas in previous studies on a slightly different PCL/PLA material MSC could only be detected until day 8. More MSC were found in wounds treated with PCL/PLA-1 carriers than in wounds treated with FISH skin carriers 14 and 21 days post wounding. In wounds treated with PCL/PLA-2 carriers AMSC were detectable until day 3 post wounding. These promising results support the application of PCL/PLA-1 and FISH skin material in combination with MSC in further wound healing studies.