Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Gassner, R.
Chemokine Receptor Expression Profile in Malignant Melanoma and Brain-Specific Metastases
Humanmedizin; [ Diplomarbeit ] Medizinische Universität Graz; 2023. pp. 41
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- Autor*innen der Med Uni Graz:
- Betreuer*innen:
- Aigelsreiter Ariane
- Leoni Marlene
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- Abstract:
- Background Cutaneous malignant melanoma is a common and potentially fatal malignancy in industrial countries, the developement of metastases is associated with poor prognosis. One of many mechanisms involved in its developing process is the expression of chemokine receptors. Since expression patterns of different chemokine receptors in the developement of brain metastases in primary malignant melanomas are still not known exactly, this thesis aimed on that issue. Furthermore, results in that field could be gate opening regarding the establishment of new therapeutical opportunities. Methods This thesis‘ target was to find out differences in expression patterns of chemokine receptors in primary malignant melanomas and their brain specific metastases. In total nine samples of primary melanomas and 25 samples of brain specific metastases as well as corresponding surrounding tissues were analysed regarding their chemokine expression patterns by using RT-qPCR. Results Five chemokine receptors (CCR4, CCR6, CCR9, CXCR4 and CXCR6) showed consistent expression patterns. All three CC chemokine receptors showed a decreased expression in melanomas in relation to control tissues as well as to metastases. Comparing CC chemokine receptor expression in melanomas and their surrounding skin tissue, significantly elevated levels of chemokine gene products were detected in melanoma‘s surrounding tissue (p < 0.05). Overall, CXCR4 was expressed in lower levels in all samples compared to controll tissue. These results resembled to those of CXCR6. Conclusion Chemokine expression patterns in melanomas are different to those in brain specific metastases. Furthermore, the chemokine profile is significantly different in primary melanomas compared to their surrounding skin tissue.