Medizinische Universität Graz - Research portal
Selected Publication:
Domingo, M.
Dissecting a novel p53-miRNA axis in brown adipocytes.
[ Diplomarbeit/Master Thesis (UNI) ] Universität Graz; 2022.
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- Authors Med Uni Graz:
- Advisor:
- Prokesch Andreas
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- Abstract:
- Cold-activated brown adipose tissue (BAT) dissipates high amounts of glucose and fatty acid as substrate to generate heat. Thus, non-shivering thermogenesis is highly energy consuming and defines BAT as a metabolic sink. However, how this process is regulated under fasting conditions, when energy needs to be conserved, is still elusive. Previous data from the lab showed that p53 signaling is the top enriched pathway and miR92a the most induced miRNA in BAT of fasted mice kept below thermoneutrality. Bioinformatics analysis predicted a direct binding of p53 at the miR92a locus and in vitro experiments highlighted fructose transporter Slc2a5 as a potential downstream target. p53 has previously been identified as a regulator of BAT activity, thereby we aimed to further investigate this axis as potential energy conserving mechanism upon fasting. The direct binding of p53 on the miR92a locus was examined by Chromatin Immunoprecipitation-quantitative PCR (ChIP-qPCR). Furthermore, the interaction between miR92a and the 3’ untranslated region (3’UTR) of Slc2a5 was investigated by luciferase assay. Our results suggest a direct binding of miR92a to the Slc2a5 mRNA and a slight enrichment of p53 binding to the miRNA92a locus. However, the ChIP protocol for validating the interaction between p53 and the miR92a locus needs further improvement.