Medizinische Universität Graz - Research portal
Selected Publication:
Wurzer, LS.
Interrelation between mitochondrial calcium and ROS signaling.
[ Diplomarbeit/Master Thesis (UNI) ] TU Graz; 2021.
FullText
- Authors Med Uni Graz:
- Advisor:
- Madreiter-Sokolowski Corina
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- Abstract:
- Calcium (Ca2+) and reactive oxygen species (ROS) are signaling molecules essential for coordinating physiological and many pathophysiological processes. Ca2+ plays an essential role in muscle contraction, signal transduction, and neurotransmitter release. To maintain Ca2+ homeostasis, the cellular and organellar Ca2+ uptake and extrusion have to be precisely regulated and balanced. Most endogenous ROS evolve from a leaky electron transport chain (ETC) during oxidative phosphorylation in mitochondria. ROS can either operate as signaling molecules or cause oxidative stress to the cell depending on the type, amount, and reactivity. It has been proposed that there is a mutual interplay between the mitochondrial Ca2+ ([Ca2+]mito) homeostasis and ROS production. However, the correlation between [Ca2+]mito and ROS production is still poorly understood. The study aimed to further elaborate how [Ca2+]mito and ROS are intertwined. Therefore, we investigated how specific modulation of [Ca2+]mito and ROS homeostasis affect each other during in vitro and in vivo aging. The interrelation between [Ca2+]mito modulation and ROS production in different cellular compartments were examined in HeLa cells by fluorescent microscopy and mRNA expression comparison. To evaluate the impact of Ca2+ and ROS homeostasis on health and lifespan in vivo, the model organism Caenorhabditis elegans (C. elegans) was used. (...)