Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Leitinger, D.
Chemokine Receptor Expression Profile in Non-Small-Cell Lung Cancer and Brain-Specific Metastases
Humanmedizin; [ Diplomarbeit ] Medizinische Universität Graz; 2022. pp. 44
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- Autor*innen der Med Uni Graz:
- Betreuer*innen:
- Aigelsreiter Ariane
- Leoni Marlene
- Altmetrics:
- Abstract:
- Background Lung cancer is one of the most common cancers in industrial countries and the leading cause of cancer-related mortality. Especially the development of brain metastases is accompanied by a poor prognosis. Since lung cancer is the tumor with the highest rate of metastasis into the brain, it is important to elucidate the causes and events in the development of metastases. Chemokines and their receptors play a key role in the formation of metastases, but also in disease progression. New insights in this field may yield new concepts in developing targeted therapies for lung cancer and their metastases. Methods This thesis aimed to find out if the chemokine receptor expression in Non-Small-Cell Lung Cancer (NSCLC) is different between the primary tumors and brain-specific metastases. Differences in the expression of the most well-characterized chemokine receptors (CCR1-10, CXCR1-7, XCR1 und CX3CR1) were detected by RT-qPCR. The chemokine receptor expression was analyzed on 13 NSCLC samples and 30 samples of brain-specific metastases from NSCLC. Furthermore, the chemokine receptor expression in tumor tissues was compared to the chemokine receptor expression in the surrounding non-neoplastic tissues. Results The CC chemokine receptors CCR4, CCR6, CCR8, and CCR9 and the CXC chemokine receptors CXCR4 and CXCR6 were consistently expressed on both the primary tumor tissues and tissues of metastases. The mRNA expression of CCR6 was significantly lower in metastases compared to the primary tumors (P=0.0429), as was the expression for CCR9 (P=0.0499). The chemokine receptor expression was also lower in metastases compared to the primary tumors for CXCR4 (P=0.0107) and CXCR6 (p=0.034). Conclusion The mRNA expression profile for some chemokine receptors is different in brain-specific metastases of NSCLC compared to the primary tumors.