Medizinische Universität Graz - Research portal
Selected Publication:
Stojanovic, S.
In vivo Investigation of Gemcitabine-IPs: Effects on the rate of C6-Glioblastoma rat cell proliferation in the Chicken Chorioallantoic Membrane Model
Humanmedizin; [ Diplomarbeit ] Medizinische Universität Graz; 2022. pp. 52
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- Authors Med Uni Graz:
- Advisor:
- Patz Silke
- Waldherr Linda
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- Abstract:
- The Glioblastoma Multiforme (GBM) is the most frequently occurring and simultaneously most malignant tumor of the brain. His rapid and highly invasive growth behaviour, the difficulty of complete tumor removal and the lack of effective therapeutical options make him one of the deadliest neoplasms in general. The current therapy for GBM consists of maximal safe tumor resection followed by adjuvant involved field radiation and chemotherapy with temozolomide. Despite a multimodal treatment approach, the overall survival and prognosis did not show significant improvement in recent decades. A very promising attempt to improve therapy success is a drug delivery device for local treatment. The specially developed iontronic pump could be implanted into the surgical cavity postoperatively and deliver substances directly to the targeted area by induction of an electromagnetic field. To establish such a concept for human patients, many intermediate steps are required before. The aim of the accomplished experimental series is to investigate the effects of a gemcitabine iontronic pump on C6 rat glioblastoma cells in the chicken chorioallantoic membrane (CAM) model. The CAM assay provides a cost-effective and easily reproducible in-vivo model for cancer research. The C6 rat cells remain the gold standard in glioma research due to several advantages. The C6 cells were xenografted on the CAM by the help of silicon rings, received different treatment conditions and subsequently the cultured tumors were harvested. A histological and immunohistochemical analysis followed. To examine the effects of the gemcitabine iontronic pump, the Ki-67 proliferation index of the different treated tumors was determined and statistically evaluated.