Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Helmer, A.
Stem cells in wound healing
Humanmedizin; [ Diplomarbeit ] Medical University of Graz; 2021. pp. 63
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- Autor*innen der Med Uni Graz:
- Betreuer*innen:
- Lang-Olip Ingrid
- Tuca Alexandru
- Altmetrics:
- Abstract:
- Amnion derived mesenchymal stroma cells (=AMSC) are an intensely studied and, yet just partially understood, ubiquitous applicable hope in medicine. Their anti-inflammatory, neo-vascular and cell stimulating properties, promoting wound healing, have been proven in many different studies. The objective of this study was to find the best possible application material for AMSC promoting their curing potential in an in vivo mouse model. In total we worked with 16 mice in two study cycles in which each mouse got two 8mm punch biopsy-wounds administered on their back. In the pilot study we compared different combinations of Matriderm®, Matrigel® and PCL/PLA with or without AMSC. In the second cycle we established a control group which we left untreated. In both rounds we gathered blood-samples, took photos for macroscopic assessment, and excised the wounds for microscopic evaluation. Additionally, we used ultrasonography on one mouse for additional insight concerning the wound-healing process. In the Pilot study all mice were sacrificed on the 14th day. Whereas, in the first experimental run 3 mice were sacrificed on the 3rd, on the 7th and on the 14th day to gain data on the inflammation histologically and from the blood parameters. Wounds treated with Matriderm®+ Matrigel®, Matriderm®+ Matrigel® + AMSC and PCL/PLA+ Matrigel®+ AMSC showed an accelerated wound healing macroscopically and a further progressed re-epithelialization under microscopic evaluation. Additionally, the infection parameters from the blood-samples taken from the wounds treated with AMSC are lower compared to the control group and the infection seems to end on the 7th day. Concluding, Matriderm® and PCL/PLA seem to be promising carrier for AMSC. Additionally, Matrigel® seems to have a beneficial effect on wound healing and AMSC. Further studies need to be done to determine the most promising application material with statistical significance and to advance to clinical trials.