Medizinische Universität Graz - Research portal
Selected Publication:
Eylert, G.
Skin regeneration and wound healing after mesenchymal stromal/stem cell treatment is dose dependent
Doktoratsstudium der Medizinischen Wissenschaft; Humanmedizin; [ Dissertation ] Medical University of Graz; 2021. pp. 100
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- Authors Med Uni Graz:
- Advisor:
- Kamolz Lars-Peter
- Lang-Olip Ingrid
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- Abstract:
- Background: Skin regeneration and wound healing is crucial, especially after a burn injury. Mesenchymal stromal cell (MSC) therapy is under investigation in promising (pre)clinical trials to restore the damaged skin; however, the optimal cell dosage for therapy remains unknown. Hypothesis: Different low-to-high MSC dosages promote outcome measures differently in skin regeneration and wound healing. Methods: We conducted a porcine study (N = 8 Yorkshire pigs) and seeded between 200–2,000,000 cells/cm2 of umbilical cord mesenchymal stromal cells (UC-MSCs) on a biodegrada-ble collagen-based dermal regeneration template (DRT) Integra® and grafted it onto full-thickness burn excised wounds. On day 28, we compared the different low-to-high cell dose groups, the acellular control, a burn wound, and healthy skin. Result: We found that the low dose with 40,000 cells/cm2 regenerates the full-thickness burn excised wounds most efficaciously, followed by the dose groups 5,000 cells/cm2 and 200,000 cells/cm2. The low dose of 40,000 cells/cm2 accelerated re-epithelialization, reduced scarring, regenerated epidermal thickness superiorly, enhanced neovascularization, reduced fibrosis and reduced type 1 and type 2 macrophages compared to other cell dosages and the acellular control. Importance: This regenerative cell therapy study using MSCs shows efficacy when utilizing a low dose, which changes the paradigm that more cells lead to better wound healing outcome.