Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Gabalier, S.
Effect of TiAl6V4 surface modifications on the expression of matrix metalloproteinases and their inhibitors.
Humanmedizin; [ Diplomarbeit ] Graz Medical University; 2020. pp. 53
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- Autor*innen der Med Uni Graz:
- Betreuer*innen:
- Lohberger Birgit
- Sadoghi Patrick
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- Abstract:
- Aseptic loosening of implanted prostheses in orthopedic surgery, which is a frequent reason for their failure, is not only attributed to inadequate initial fixation or mechanical loss of fixation over time, but also to osteolysis observed in the surrounding bone. It is currently assumed that this osteolysis in the area surrounding implants is caused both by particles produced by means of mechanical abrasion of the prosthesis material and by metal ions dissolved by electrochemical processes. According to our hypothesis, the expression of genes whose products are essential for the renewal of the extracellular matrix of bone tissue and thus for the maintenance of its mechanical function is altered in osteoblasts if these cells are in direct contact with TiAl6V4-Implants, whose surfaces have been modified in different ways (TiAl6V4+TiN, TiAl6V4+Ag, TiAl6V4-roughening and TiAl6V4+cpTi). This could possibly contribute to aseptic prosthesis loosening. Therefore we analysed the impact of this TiAl6V4 surface modifications on both cytotoxicity and gene expression of matrix metalloproteinases (MMP2, MMP9, MMP14) and their inhibitors (TIMP1, TIMP2) as well as the expression of Collagen type I (COL1), Osteopontin (SPP) and Osteocalcin (BGLAP) in osteoblasts (hFOB). Compared to TiAl6V4, our investigations did not show any significant increase in cytotoxicity for the modified surfaces we investigated. Furthermore, it has been found that the gene expression of MMP9 and MMP14 was generally very low, and with the exception of the group TiAl6V4+Ag no significant differences in the expression of MMP2, TIMP1, TIMP2, COL1, SPP or BGLAP by hFOB cells growing on the modificated surfaces of TiAl6V4 we investigated (TiAl6V4+TiN, TiAl6V4-roughening and TiAl6V4+cpTi) could be found compared to TiAl6V4. From that it can be concluded that the above mentioned surface modifications do not have any significant influence on the expression of MMP2, TIMP1, TIMP2, COL1, SPP or BGLAP by osteoblasts and thus do not, or at least not by this pathway, contribute to aseptic prosthesis loosening. The cause of the significantly different expression of MMP2, TIMP1 and SPP in hFOB cells growing on TiAl6V4+Ag compared to the group TiAl6V4 and whether or not there is a relationship between these changes in the expression of those genes could not be clarified in the present diploma thesis and will require further investigations.