Medizinische Universität Graz - Research portal
Selected Publication:
Arneitz, C.
Cardiorespiratory Performance Capacity and Pulmonary Microbiome in Patients Following Surgical Repair of Esophageal Atresia.
Doktoratsstudium der Medizinischen Wissenschaft; Humanmedizin; [ Dissertation ] Graz Medical University; 2020. pp. 101
[OPEN ACCESS]
FullText
- Authors Med Uni Graz:
- Arneitz Christoph
- Advisor:
- Castellani Christoph
- Singer Georg
- Altmetrics:
- Abstract:
- Introduction: Patients following esophageal atresia (EA) repair more frequently suffer from respiratory symptoms and impairment of cardiopulmonary function. Possible underlying reasons may be alterations of the airway microbiome. The aim of this prospective cohort study was to investigate the cardiopulmonary performance capacity and the airway microbiome of adolescent and adult patients after corrected EA and to compare the findings to healthy age- and sex-matched controls. Methods: All EA patients treated between 1980 and 2010 were invited to a prospective study consisting of clinical examination, conventional spirometry and exhausting bicycle spiroergometry. The airway microbiome was determined from deep induced sputum by 16S rRNA gene sequencing. The results were compared to a healthy age- and sex matched control group. Results: Nineteen EA patients with a mean age of 24.7 years and 19 age- and sex-matched controls were included. EA patients showed a significantly lower muscle mass compared to the control group. Spirometry revealed a significantly lower VCmax and higher rates of restrictive ventilation disorders of EA patients; significantly lower relative performance capacity and a significantly lower peak VO2 were found in spiroergometry. Alpha- and beta-diversity of the airway microbiome did not differ significantly between the two groups. LEfSe analysis revealed significantly enriched species of Prevotella uncultured, Streptococcus anginosus, Prevotella 7 Prevotella enoeca and Mogibacterium timidum in the EA patients. Conclusion: In the long-term outcome, EA patients frequently suffer from restrictive ventilation disorders and impaired cardiopulmonary function associated with discreet alterations of the airway microbiome. Long-term examinations of EA patients consisting of routine spirometry and spiroergometry seem to be necessary in order to detect impaired cardiopulmonary function and to prevent the progression of associated complications.