Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Fuchs, MT.
The Association of Human Milk Oligosaccharides with Glucose Metabolism in normal weight pregnant women.
[ Diplomarbeit/Master Thesis (UNI) ] Universität Graz; 2020. pp.34.
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- Autor*innen der Med Uni Graz:
- Weiser-Fuchs Marie-Therese
- Betreuer*innen:
- Jantscher-Krenn Evelyn
- Wadsack Christian
- Altmetrics:
- Abstract:
- Background: Human milk oligosaccharides (HMOs) are structurally diverse bioactive factors in breastmilk, also present in maternal blood during pregnancy. A previous study in obese/overweight women suggests that sialylated HMOs in early pregnancy influence maternal metabolism during gestation. Whether specific HMOs are associated with glucose metabolism markers also in normal weight women, is unknown. Hypothesis: We hypothesized that HMOs in early pregnancy are associated with glucose metabolism and insulin sensitivity in later pregnancy. Methods: Serum samples were available at three time points (12, 20 and 33 gestational weeks) in pregnancy and at delivery (48h postpartum) from a cohort of 120 healthy women. HMOs were isolated by solid phase extraction and quantified by HPLC analysis. Serum glucose, insulin, C-peptide, GLP-1, leptin and adiponectin were measured by conventional methods. HOMA index was calculated from fasting glucose and insulin at oGTT. Spearman correlations were performed to investigate associations between specific HMOs and metabolic parameters. Results: 18 HMOs were identified in maternal serum, and their concentrations increased over gestation. The four most abundant HMOs 2’FL, 3’SLN, LDFT and 3’SL in early pregnancy were associated at follow-up visits. Cross-sectional analyses revealed significant positive associations of 3’SL and 3’SLN with GLP-1, C-peptide, leptin and insulin. 3’SL and 3’SLN concentrations in early pregnancy were associated with GLP-1 and leptin at follow-up visits and with HOMA index at 28 weeks. Conclusion: This study confirmed presence and temporal variations of HMOs in maternal serum during pregnancy, and found positive associations of sialylated HMOs 3’SL and 3’SLN early in pregnancy with markers of glucose metabolism and insulin sensitivity. This suggests potential roles for sialylated HMOs in modulation of glucose metabolism also in normal weight pregnant women, warranting further research on causal relationships.