Medizinische Universität Graz - Research portal
Selected Publication:
Friehs, E.
Exhaled Volatile Organic Compounds during Pediatric Open and Laparoscopic Appendectomy – A Prospective Randomized Pilot Study.
Humanmedizin; [ Diplomarbeit ] Graz Medical University; 2020. pp. 66
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- Authors Med Uni Graz:
- Advisor:
- Singer Georg
- Warncke Gert
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- Abstract:
- Introduction: Acute appendicitis is one of the most frequent causes of an abdominal emergencies in children and adolescents. Appendectomies can be performed either open or laparoscopically. However, it is still unclear which of the two methods releases less oxidative stress. Analyzation of volatile organic compounds (VOCs) from exhaled breath samples offers the possibility to monitor oxidative stress levels intraoperatively. Methods: In this prospective randomized pilot study, VOCs in exhaled breath of fourteen children were sampled during an open (n=7) or a laparoscopic (n=7) appendectomy at five different time points (after induction of anesthesia, 15 min, 30 min, 45 min after the first cut and after the last suture). Acetone, isoprene, dimethylsulfide and benzene were chosen as oxidative stress markers and analyzed by gas chromatography and mass spectroscopy at the Technical University Graz. Results: A significant difference in concentration levels of acetone between the two surgical methods at the first (induction of anesthesia) and second (15 min after the first cut) sampling points was seen. The concentration of dimethylsulfide was significantly different between the two approaches at the first three (after induction of anesthesia, 15 min, 30 min after the first cut) and the last (after the last suture) sampling points. Isoprene and benzene did not show significant concentration changes during the sampling points. There was a significant difference of the benzene level during laparoscopic appendectomies, while it displayed no significant change during open appendectomies. The concentration level of isoprene, acetone and dimethylsulfide showed no significant differences comparing the two methods. Conclusion: Significant changes of VOC concentrations in breath samples between open and laparoscopic appendectomies were seen despite the small sample size number. Further studies with a larger number of patients are, however, necessary to verify the results.