Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Spies,M.
Characterisation of the expression profile of pattern recognition receptors in TGF-β1- vs. BMP7-driven Langerhans cells
Humanmedizin; [Diplomarbeit] Medical University of Graz;2019. pp. 53
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- Autor*innen der Med Uni Graz:
- Betreuer*innen:
- Strobl Herbert
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- Abstract:
- Langerhans cells (LCs) are dendritic cells (DCs), which reside in the epidermis and play an important role in the adaptive immunity. LCs exhibit a unique marker profile, which enables their precise distinction from other DCs (e.g. CD1a+CD207+CD324+). They also express Toll like receptors (TLRs), which are pattern recognition receptors important for the detection of pathogens. The role of LCs in the regulation of the immune system responses is still debated as their behavior is heavily context dependent: LCs can be immunosuppressive as well as they can induce an inflammatory and cytotoxic T-cell response. Transforming growth factor beta 1 (TGF-β1) and bone morphogenetic protein 7 (BMP7) are cytokines that belong to the TGF-beta family composed out of structurally related, cell regulatory proteins. We recently found that BMP7 fully replaced TGF-β1 in LC generation cultures. The aim of this study was to characterize and compare the TLRs expression pattern of BMP7-dependent LCs vs. TGF-dependent LCs. qPCR analysis reviled that BMP7-generated LCs expressed significantly higher levels of TLR2 compared to TGF-β1-generated LCs. In contrast TGF-β1-generated LCs expressed higher levels of TLR1, TLR6 and TLR10 than BMP7-generated LCs. Both cell types had no detectable expression of TLR4, TLR5 and TLR9. To compare LCs to other DCs, we additionally analyzed the TLR expression profile of CD14+ monocyte derived dendritic cells (MoDCs). We showed that MoDCs express TLR1, TLR2, TLR3, TLR4, TLR6 and TLR8 and they had no detectable expression of TLR5, TLR7, TLR9 and TLR10. Stimulation with lipopolysaccharide (LPS) resulted in downregulation of all analyzed receptors.