Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Kornhaeusel,G.
The Chick Chorioallantoic Membrane as an In-Vivo Xenograft for Human Skin in Regenerative Medicine - A model for studying ischemic wounds and the regeneration process of CAM integrated skin grafts
Humanmedizin; [Diplomarbeit] Medical University of Graz;2019. pp. 74
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- Autor*innen der Med Uni Graz:
- Betreuer*innen:
- Smolle Christian
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- Abstract:
- Introduction: Wound healing is a complex process consisting of several phases and requiring an ideal interaction between cellular and biochemical factors. An essential step of this regenerative process is adequate supply of the wound area with oxygen and nutrients by neovascularisation. This enables cell proliferation and biochemical synthesis procedures to renew tissue. Ischemia or simply inadequate blood supply of tissues can lead to the formation of chronic wounds. Approximately 1 percent of the population in Germany suffer from chronic wounds, incidence and prevalence increasing (1). Therefore, a number of studies are needed to develop models, which can be used for studying the complexity of the regeneration process of human skin (3). This understanding will lead to new products and therapies for patients. With this diploma thesis we aim to establish methods to develop a new model for studying ischemic wounds and the regeneration process of human skin based on chick chorioallantoic membrane integrated split skin grafts. Material and Methods: Chick chorioallantoic membrane assays were cultured following an established ex-ovo method from Deryugina et.al. On day 7 split skin grafts were transferred onto the CAM. After 3 days full integration of the xenografts into the CAM was detected. Using a special pulled micropipette 3 µL of a 50mg/mL solution of Rose bengal was applied intravenously and by means of a 120W cold light lamp and a 525/50nm filter the photothrombosis was induced after the selection of an appropriate CAM area. Results: The results obtained in the diploma thesis indicate that grafting of human skin on the chick chorioallantoic membrane, the injection of Rose bengal in CAM blood vessels, the haemostasis of bleeding and the induction of photothrombosis are successfully implemented. A typical pattern of vessel changes could be observed. After 8min of illumination an obvious reduction of vessel density and a visible decrease of the vessel lumen could be seen. Discussion: The follow-up of ischemic wounds was not yet possible, since the chicken embryos only survived hours after treatment. We assume that this may be because of a certain dosage dependent toxicity of Rose bengal in the developing chicken embryo or the blood loss – some millilitres - appearing after the injection process.