Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Weller, A.
Detection of extracellular vesicles in Chordoma cell lines-Examination of extracellular vesicle occurrence and behaviour.
[ Diplomarbeit/Master Thesis (UNI) ] University of Graz; 2018. pp.63.
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- Autor*innen der Med Uni Graz:
- Betreuer*innen:
- Rinner Beate
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- Abstract:
- Chordoma is a low to intermediate grade bone tumor occurring at the axial skeleton caused by malignant transformation of embryonic notochord remnants. The anatomical location of the tumor is mostly at the sacrum and clivus. The current therapy includes the surgical resection of the affected tissue and a chemo-and radiotherapy. The challenging location of the tumour, the resistance of chordoma cells against radiotherapy and chemotherapy and a high local recurrence rate are common difficulties for a promising healing process. The sum of difficulties indicates the major need of alternative strategies for chordoma treatment. Extracellular vesicles (EVs) were reported as potential drug-carriers in cancer-treatment, e.g. melanoma. EVs are membrane-encapsulated particles, distributed from almost all cells. Several types of EVs were mentioned in literature and classified in 3 groups depending on their subcellular origin and biogenesis: Exosomes (30-120 nm), microvesicles (100-1000 nm) and apoptotic bodies (1000-5000 nm). To investigate the distribution of chordoma-derived EVs MUG-Chor1, a sacral patient derived chordoma cell line, and MUG-CC1, a clival patient derived chordoma cell line were analysed by physical characterization methods, like flow cytometry and Nanoparticle tracking analysis. The investigations proved the evidence of EV release in both chordoma cell lines. Herby, both prefer to release exosomes, in different size ranges. MUG-Chor1 distributes smaller exosomes compared to MUG-CC1. The presences of exosomes were evidenced by tetraspanin marker CD81. Furthermore, the influence of hypoxic conditions showed a tendency for increased EV-release compared to normoxic conditions. Moreover, for preliminary experiments the strong proliferating RFP-tagged MUG-Mel1 cell line was used. The purified RFP-tagged MUG-Mel1-derived EVs showed a co-localization in their parent cell and in fibroblast, which could be an indication for bioactive EVs after isolation. In conclusion, it can be said that chordoma cell lines are able to produce nano-sized EVs, which could act as potential drug carriers.