Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Scheiber, O.
Evaluation of intracytoplasmic hyaline inclusions as markers for malignancy in hepatocellular liver tumors
Humanmedizin; [ Diplomarbeit ] Graz Medical University; 2018. pp. 69
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- Autor*innen der Med Uni Graz:
- Betreuer*innen:
- Aigelsreiter Ariane
- Lackner Karoline
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- Abstract:
- Background and aims: Hyaline bodies, Mallory-Denk bodies (MDB) and intracellular hyaline bodies (IHB), may occur as a consequence of a variety of stresses in cells with hepatocellular differentiation. MDB have been described in benign liver diseases as well as in benign and malignant tumors of the liver. In contrast, IHB have only been observed in hepatocellular carcinomas (HCC). Thus IHB may serve as morphological tumor markers. Therefore, the aim of this study is to analyze occurrence of these intracytoplasmic hyaline inclusions in precursor lesions of HCC in cirrhotic and non-cirrhotic liver. Patients, materials and methods: 61 precursor lesions, 11 large cell changes, 10 small cell changes, 20 low-grade dysplastic nodules (LGDN), 13 high-grade dysplastic nodules (HGDN), 7 HGDN with HCC foci, 90 HCC and additionally, benign tumors with malignant potential, 23 focal nodular hyperplasias (FNH) and 13 hepatocellular adenomas (HCA), were histologically as well as immunohistochemically analyzed and occurrence of MDB and IHB was correlated with histological diagnosis by statistical analysis. Results: In our study, MDB were identified in all precursor lesions in cirrhotic liver and in regenerative nodules of cirrhosis. Highest relative frequencies have been observed in HGDN (53.8%) and in HGDN with foci of HCC (71.4%). In contrast, IHB have exclusively been detected in 31.1% of HCC and in 28.6% of HCC foci within HGDN but in none of the other precursor lesions of HCC. Conclusions: IHB and MDB can be easily and inexpensively identified on H&E histology. Occurrence of IHB in tumors of the liver with hepatocellular differentiation correlates strictly with malignant hepatocellular lesions and thus IHB are able to support diagnosis of HCC as a tumor marker whereas MDB in a dysplastic nodule may suggest high-grade dysplasia.