Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Binder, C.
Impact of mesenchymal stem cells on the endothelialisation of vascular grafts.
[ Diplomarbeit/Master Thesis (UNI) ] Graz University; 2017. pp.61.
- Autor*innen der Med Uni Graz:
- Betreuer*innen:
- Brislinger Dagmar
- Altmetrics:
- Abstract:
- The need of synthetic vascular grafts increase due to the aging of the society. Non-biodegradable ePTFE grafts are currently used as large calibre vascular prosthesis but they fail as small calibre graft (< 6 mm). Biodegradable PCL/PLA grafts represent a promising alternative for microvascular surgery. Small calibre vascular grafts represent a challenge to material scientists because they are prone to thrombogenic complications. A strategy to minimise the problem of graft occlusion is represented by seeding endothelial cells from the human placenta (hPEC) on the luminal graft surface. Amnion-derived mesenchymal stem cells (hAMSC) could improve endothelial properties with their angiogenic and immunomodulatory potential. The purpose of our study was to investigate the adhesion and proliferation of hAMSC and hPEC on PCL/PLA vascular grafts. Additionally, single and co-culture long-term experiments were carried out in a 3D co-culture model (BC - Bioreactor) to investigate the impact of hAMSC on the endothelialisation of PCL/PLA vascular grafts. We showed that PCL/PLA had no impact on cell growth or proliferation of hAMSC and hPEC. Light microscopic evaluation determined an improved cell adhesion of hAMSC on fibronectin pre-coated PCL/PLA grafts compared to the native graft material. A possible migration of hAMSC was examined by a migration assay and additional by light microscopic analysis of cross sections of the paraffin embedded membrane. We showed that the migration of hAMSC into the PCL/PLA graft is time dependent. The cells passed three-quarters of the membrane 5 days after seeding. hPEC in single culture exposed to flow (up to 7 dyne/cm2) show an increased cell growth on the inner graft surface in the BC - Bioreactor, compared to static conditions. In co-culture with hAMSC, the inner graft surface was also coated with a confluent hPEC layer. On the outer graft surface were only few hAMSC attached.