Medizinische Universität Graz - Research portal
Selected Publication:
Forstner, D.
Influence of platelet-derived factors on the villous trophoblast.
[ Diplomarbeit/Master Thesis (UNI) ] Graz University; 2017.
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- Authors Med Uni Graz:
- Advisor:
- Gauster Martin
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- Abstract:
- During the whole pregnancy, the placenta plays a central role, because it is responsible for the exchange of gasses and nutrients between maternal and fetal side, as well as for the production of hormones. Platelets adhere during the pregnancy on the villous trophoblast and influence it on different levels. This influence is investigated during the master thesis. The endocrine activity of the cells regarding the hormone hCG as well as their viability and proliferation is focused. Furthermore, changes in the cells morphology and fusion are investigated. The processing of different tissue samples as the BeWo cell line and explant cultures of early and term placenta, as well as the transaction of ELISA, LDH assays, BrdU assays, qPCR, western blots and immunohistological techniques and subsequent light- and fluorescence microscopy are belonging to the responsibilities in the lab. An influence of platelets on the villous trophoblast regarding the fusion and endocrine activity was observed. The hCG concentrations decreased on protein as well on gene expression level. Furthermore, a decrease in the cell fusion due to the addition of human platelet lysate could be established. The proliferation behavior, the viability and the cells morphology remain most uninfluenced. On the basis on the generated results during my lab work, there is a hint, that the differentiation of the villous trophoblast to multinucleated syncytium is eventually hampered by pHPL however. The results of placenta samples varied because of many influent parameters during pregnancy, like the BMI and gestational week. The fact, that many results from BeWo experiments could be verified in first trimester placenta explants, was also very interesting.