Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Pincus, M.
The TGF-Beta Signaling Pathway in Malignomas
Humanmedizin; [ Diplomarbeit ] Graz Medical University; 2017. pp. 36
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- Autor*innen der Med Uni Graz:
- Betreuer*innen:
- Gürtl-Lackner Barbara
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- Abstract:
- Activation of the TGF-ß pathway plays a pivotal role in human tumorigenesis, acting either as tumor suppressor or showing oncogenic characteristics. MicroRNAs (miRNAs) are small non coding RNAs, which regulate gene expression by post transcriptional mechanisms. Activation of the TGF-ß signalling pathway by deregulation of miRNAs has already been described in nephroblastomas. In our study we therefore investigated several miRNAs that influenced gene activation of the TGF-ß signalling pathway in an in silico analysis. Target genes and miRNAs of the TGF-ß pathway were analysed by the DIANA mirPath programme, which is a tool to identify molecular pathways targeted by single or multiple miRNAs. Expression levels of miRNAs were investigated in different subtypes of nephroblastomas by quantitative REALtime PCR. Above that we investigated the influence of single microRNA on their predicted target genes in a cell culture experiment. The in silico analysis predicted miR 27a, miR 145 and miR 193 5p to target several genes within the TGF ß signalling pathway. Samples analysed in previous studies showed a lower expression of miR 27a whereas in this study no deregulation of miR 145 and miR 193 5p could be detected. Furthermore the influence of single microRNA on predicted TGF ß genes could not be confirmed. Our results therefore indicate a different regulation of the TGF ß genes in nephroblastomas.