Medizinische Universität Graz - Research portal
Selected Publication:
Fischbach, L.
Parallel detection of (1¿3)-ß-D-Glucan in corresponding same day urine and serum samples obtained from patients with hematological malignancies
Humanmedizin; [ Diplomarbeit ] Graz Medical University; 2016. pp.
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- Authors Med Uni Graz:
- Advisor:
- Rabensteiner Jasmin
- Raggam Reinhard Bernd
- Altmetrics:
- Abstract:
- Background: Serum 1,3-beta-D-glucan (BDG) testing is an diagnostic marker for invasive fungal infections (IFI) applied for patients with hematological malignancies. Though for the application of BDG urine testing, currently limited data exists. Methods: Serum and urine samples were collected on the same day, twice a week in parallel, from adult patients with underlying hematological malignancies. A total of 241 urine samples from 60 patients were investigated with the BDG Fungitell® assay. In addition, a BDG/Creatinine ratio to exclude urine dilution bias was introduced. Results: 19 had positive corresponding serum BDG (>200 pg/ml), 33 intermediate (80-200 pg/ml), and 189 negative serum BDG (<80 pg/ml). Sensitivity, specificity, positive predictive value and negative predictive value (according to the patients state of IFI) were: 32%, 84%, 50%,70% when using an 80 pg/ml urine cut-off, and 20%, 95%, 67%, 70% using a 200 pg/ml cut-off. With the urine BDG/Creatinine ratio, the results showed higher sensitivities ranging from 47-81% without significant loss of specificity, which ranged from 49% to 70%. Correlation was superior in the groups >200 pg/ml (BDG urine: r=0.453; p=0.051; urine BDG/Creatinine ratio: r=0.722; p=0.000) compared to the negative and intermediate ranges. Conclusion: Overall correlation of same-day urine BDG and serum BDG was moderate. In general correlation and the clinical performance was superior when the urine BDG/Creatinine ratio was applied, therefore urine BDG testing in urine could be improved by introducing a BDG/Creatinine ratio.