Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Tuca, A.
Evaluation of Arterial Blood Glucose Measurements in a Porcine Model
Humanmedizin; [ Diplomarbeit ] Graz Medical University; 2016. pp.
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- Autor*innen der Med Uni Graz:
- Tuca Alexandru
- Betreuer*innen:
- Korsatko Stefan
- Pieber Thomas
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- Abstract:
- Introduction: Glucose control is of importance for diabetic patients at home, but according to recent findings also for patients in the hospital or for critically ill patients suffering from stress induced hyperglycaemia. Over the last decades, substantial effort has been made towards the development of glucose biosensors that can be efficiently and safely used in different settings. Due to increased safety and quality demands in health care it is necessary to evaluate these glucose biosensors in a safe and effective preclinical setting. This setting should be able to test the sensors’ accuracy in different glucose ranges (hypo-, normo- and hyperglycaemic) in vivo. An ideal model for this purpose could be the anesthetized porcine model. The aim of this thesis was to evaluate the feasibility of a preclinical porcine model for the investigation of blood glucose biosensors. Material and Methods: In the present thesis 10 healthy pigs underwent three different glucose and oxygen clamp protocols (A, B and C) under general anaesthesia for 10 hours. Two arterial catheters placed into the inguinal arteries were used for blood sampling to perform reference measurements and biosensor measurements. The clamp profiles were evaluated using the coefficient of variation (CV), a parameter which describes the quality of the glucose clamps. Results: In clamp protocol A, the mean CV of glucose target 100 mg/dl was 8.01%±3.78%, the mean CV of glucose target 40 mg/dl 19.65%±11.09% and the mean CV of glucose target 200 mg/dl was 8.98%±6.45%. In clamp protocol B, the mean CV of glucose target 100 mg/dl was 15.06%±19.75%, the mean CV of glucose target 40 mg/dl 8.68%±2.58% and the mean CV of glucose target 200 mg/dl was 13.23%±9.63%. Mean CV for clamp protocol C during the 10 hour normoglycemic (100mg/dl) period was 6.99%. Conclusions: The experiments indicate that the present anesthetized porcine model still needs improvements concerning the clamp quality. Nevertheless, taking these into account the present anesthetized porcine model is capable to provide an safe and effective preclinical environment for evaluation of glucose biosensors in varying glucose and oxygen ranges.