Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Bubik, S.
Cellular biocompatibility on various dental materials
Zahnmedizin; [ Diplomarbeit ] Graz Medical University; 2016. pp.
[OPEN ACCESS]
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- Autor*innen der Med Uni Graz:
- Betreuer*innen:
- Arnetzl Gerwin
- Lohberger Birgit
- Altmetrics:
- Abstract:
- Introduction: Bionic replacement of deceased body parts remains a top priority in medical research. To approve the biocompatibility of the biomaterials applied in such devices, in vitro testing is one of the first in a sequence of steps necessary to render a material fit for medical application. The three material groups used in dental prosthetics include metals, ceramics and polymers. The material of choice for dental implants is commercially pure titanium, while other materials such as zirconia and poly-ether-ether-ketone (PEEK) are considered highly promising due to their functional, biomechanical and esthetic properties. The aim of this study was to determine whether PEEK with defined mean surface roughness and composition can retrieve results equal to machined titanium or zirconia. Materials and Methods: Disks measuring 14mm in diameter and 1mm in thickness made from commercially pure titanium (cp-Ti), Yttria-stabilized zirconia (Y-TZP) and filled PEEK with specific surface finish were provided by Bredent Medical GmbH for cell culture experiments. Human fetal osteoblasts (hFOB) and human gingival fibroblasts (hGF) were cultured on each material to observe changes after 1, 3 and 7 days in cell viability (MTS assay) and lactate dehydrogenase release (Cytotox assay). Additionally mRNA expression of proliferative factors PCNA and Ki67, cell morphology (Scanning electron microscopy and Vimentin IF-staining) and cell adhesion (Vinculin IF-staining and mRNA expression) was analyzed after 3 days in culture. Results: In hFOB cultures, adhesion and viability was decreased on PEEK disks. No significant difference was observed in cp-Ti and Y-TZP when compared to control. In hGF cultures, all three materials performed almost equal, with only a slight difference in adhesion on PEEK disks in the Vinculin IF-staining. Conclusion: Commercially pure titanium and Yttria-stabilized zirconia performed similar to control in all tests and remain two equal and reliable implant materials. Highly polished PEEK in this particular composition cannot be recommended for osseointegrative applications such as dental implants due to decreased osteoblast attachment. Regarding fibroblast attachment to PEEK, usage in implant suprastructures such as abutments and dentures seems reasonable.