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Selected Publication:
Pichlsberger, M.
Application of human mesenchymal stem cells of the placenta and the umbilical cord in a murine wound healing model
Humanmedizin; [ Diplomarbeit ] Graz Medical University; 2016. pp.
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- Authors Med Uni Graz:
- Pichlsberger Melanie
- Advisor:
- Lang-Olip Ingrid
- Parvizi Daryousch
- Altmetrics:
- Abstract:
- Mesenchymal stem/stromal cells (MSC) derived from human term placenta or umbilical cord are currently objective of numerous studies concentrating on the improvement in wound care. These stem cells appear to be an ideal resource for therapeutic use in wound management due to their high differentiation potential, low immunogenicity in vivo and their good availability in large quantities. The objective of this study was to evaluate the potential of these stem cells to enhance wound healing and to examine their therapeutic usability in an in vivo mouse model. MSCs were isolated from human term placental blood vessels (bv-MSC) as well as from the Wharton’s jelly of umbilical cords (wj-MSC). Additionally, placental endothelial cells (PLAEC) from chorionic vessel explants were obtained. 18 immune deficient NSG mice were chosen for the in vivo experiment and two 8 mm punch biopsy wounds were created on each mouse back. Cells were suspended in Endothelial Growth Medium (EGM) and Matriderm Collagen Elastin Matrix® served as carrier material for MSC and EC treatment of wounds. We investigated the effects of Matriderm® supplied with EGM only compared to Matriderm® supplied with bv-MSC or wj-MSC and the effects of bv-MSC/wj-MSC in co-application with PLAEC. All animals were sacrificed at day 8 and after taking photographs for macroscopic evaluation, plugs were excised for histological examination. Single application of bv-MSC and wj-MSC showed a significant accelerating effect on wound healing by macroscopic evaluation (p<0.05), whereas additional application of PLAECs did not yield further improvement. Microscopic evaluation revealed a higher vascularization and growth of peripheral nerves in wounds treated with MSCs solely as well in those treated by bv-MSC and PLAEC. Unexpectedly, the application of wj-MSC and PLAEC did not show an improvement. In summary we conclude that the application of mesenchymal stem cells, independently of their origin, has clear beneficial effects on the healing of full thickness wounds and therefore this approach represents a promising strategy in cell based therapies of chronic wounds.