Medizinische Universität Graz - Research portal
Selected Publication:
Kamble , P.
Proteomic study to elucidate the role of lipid metabolism induced oxidative stress in cancer progression.
[ Diplomarbeit/Master Thesis ] Univ. Skövde; 2013. pp.26.
- Authors Med Uni Graz:
- Advisor:
- Birner-Grünberger Ruth
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- Abstract:
- Cellular transformation and progression of cancer is caused by mutation and epigenetic changes. These changes involve the formation of new circuits in cellular signalling and remodelling of metabolic pathways. In addition to glucose metabolism described by Otto Warburg, altered lipid metabolism also plays a significant role in tumorigenesis. In the present study, the effect of altered lipid metabolism in cancer cell proliferation was observed in adipose triglyceride lipase knock out (ATGL-KO) and wild type (Wt) murine tumor B cells. Higher growth rate of ATGL-KO cells may involve changes in reactive oxygen species (ROS) since ATGL provides fatty acids for mitochondrial beta oxidation by hydrolyzing intracellularly stored triacylglycerol. ROS assays were performed to investigate redox state of tumor B cells in order to answer the question if there are differences in ROS load between ATGL-KO and Wt tumor cells and if those differences can account for the growth advantage of ATGL-KO tumors. At basal condition, no significant difference in ROS levels between ATGL-KO and Wt was observed. However, under oxidative stress a significantly higher level of ROS was observed in Wt. Maintenance of low level of ROS in ATGL-KO under oxidative condition may involve an upregulation of antioxidant enzymes. In addition, for the comparison of basal global soluble protein expression profile of Wt and ATGL-KO tumor cells, 2D DIGE approach combined to mass spectroscopy was employed and 15 differentially expressed proteins were identified with functions in glycolysis, protein, purine and sterol biosynthesis, protein degradation and apoptosis.