Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Sinner, V.
Comparison of the multiplex eSensor® Respiratory Viral Panel to duplex real-time PCR assays for detection of viral respiratory pathogens
Humanmedizin; [ Diplomarbeit ] Medical University of Graz; 2015. pp.
[OPEN ACCESS]
FullText
- Autor*innen der Med Uni Graz:
- Betreuer*innen:
- Kessler Harald
- Altmetrics:
- Abstract:
- Background: Rapid and accurate diagnosis of acute respiratory tract infections is of major importance. Recently, the eSensor® Respiratory Viral Panel (RVP), a qualitative assay for simultaneous detection of 7 respiratory viruses and several viral subtypes has been introduced. Objectives: To evaluate the accuracy of the new eSensor® RVP assay. To evaluate the clinical and laboratory performances of the new assay and to compare results to those obtained with duplex assays routinely used. Study design: The accuracy of the eSensor® RVP assay was evaluated with panels of secondary reference material. For comparison of the clinical performances, 94 residual respiratory specimens that remained following routine clinical testing were included. Additionally, times-to-result for both testing methods were compared. Results: When eSensor® RVP accuracy was tested with proficiency panels, all results were found to be concordant with the expected results. When analyzing 94 routine samples with the eSensor® RVP, results obtained from 87 samples (92.6%) were found to be concordant with those obtained with the duplex standard assays. Seven samples (7.4%) gave discrepant results. When times-to-results were compared, the eSensor® RVP required 315 min compared to 160 min when using the routine duplex assays. Conclusion: The eSensor® RVP showed excellent accuracy and concordance with proficiency panels. Comparison of the clinical performance revealed concordant results in the vast majority of samples. When employing this multiplex assay, more pathogens can be detected simultaneously and this may have an impact on patient management.