Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Manghabati, D.
Does exercise during immobilization prevent muscle loss?
Humanmedizin; [ Diplomarbeit ] Medical University of Graz; 2014. pp. 72
[OPEN ACCESS]
FullText
- Autor*innen der Med Uni Graz:
- Betreuer*innen:
- Goswami Nandu
- Altmetrics:
- Abstract:
- Abstract Introduction: Immobilization is often seen in diseases and is accompanied with a decline of skeletal muscle mass and strength. The underlying molecular mechanisms are yet not fully understood. This diploma thesis gives an overview of the pathophysiology of skeletal muscle and the effects of immobilization and exercise on the skeletal muscle. Using OMIC technologies, data collected during an ESA bedrest were explored with focus on how exercise effects the molecular mechanism of muscular atrophy. Compared were data of bedrest immobilization with and without exercise intervention. The results have applications in cardiology, geriatrics and immobilization. Aims and Objectives: The aim of this diploma thesis is to explore how bed rest affects the muscular system of the human body, with emphasis on the underlying molecular mechanisms and if any differences can be observed depending on the type of intervention. We tested the hypothesis that exercise prevents muscle loss during bedrest immobilization and that this will be seen in the observed molecular changes. Methodology: Twelve healthy male volunteers participated in this 21-day six-degree head down bed rest ESA study at MEDES, Toulouse. Their average weight was 69.8 8 kg, their average age was 34 8 years and they had an average height of 1.76 6 cm. Blood samples taken pre- and post-bedrest where used to examine changes in the gene expression important for skeletal muscle function. Results: Eight genes KCNH2, FBXO32, IGF1, YAP1, PRSS12, SGCB, NOS1 and TRIM63 involved in muscle physiology had expression values changes with a fold change greater than +1.5 or less than -1.5. KCNH2, NOS1 and GDAP1 (down regulated) and TRIM63 and HSPB3 (up regulated) are expressed genes that are in common between the bedrest and bedrest and exercise group. Conclusions: Bedrest has an effect on gene expression. Transcriptomics can detect these changes. No gene was found that has significantly changed between the group bedrest and bedrest and exercise.